RT-PCR Based Detection of Rupestris stem pitting associated virus Within Field-Grown Grapevines Throughout the Year

Complete genome sequences of two new variants of Grapevine rupestris stem pitting-associated virus and comparative analyses.

Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus within the family Flexiviridae, is the putative causal agent of the disease Rupestris stem pitting (RSP) of grapevines. GRSPaV comprises a family of variants whose pathological characteristics have not been determined. Recently, many of the indicator "St George" plants (Vitis rupestris) used throughout ...

Sequence diversity, population genetics and potential recombination events in grapevine rupestris stem pitting-associated virus in Pacific North-West vineyards.

Grapevine rupestris stem pitting-associated virus (GRSPaV; genus Foveavirus, family Flexiviridae) is present in many grape-growing regions of the world. A total of 84 full-length coat protein (CP) sequences and 57 sequences representing the helicase-encoding region (HR) of the RNA-dependent RNA polymerase were obtained from wine grape cultivars grown in the Pacific North-West (PNW) of the Unite...

شناسایی مولکولی و گروه بندی جدایه های دو ویروسapple stem grooving virus و apple stem pitting virus در استان اصفهان

چکیده سیب درختی (malus domestica borkh) متعلق به خانواده rosaceae از میوه های مهم و اقتصادی مناطق معتدله و سردسیری ایران است. ویروس ساقه شیاری سیب apple stem grooving virus (asgv) عضو تیپ جنس capillovirus و ویروس ساقه آبله ای سیب apple stem pitting virus (aspv) عضو تیپ جنس foveavirus متعلق به خانوادهbetaflexiviridae ، از مهمترین ویروس های بیماری زای سیب محسوب می شوند. آلودگی این دو ویروس درس...

Development of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus

Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...

Field Study of Infectious Bronchitis Virus in Broiler Using Type-Specific RT-PCR